rapid determination of pathogenic bacteria in surface waters

Final Report
Rapid Determination of Pathogenic Bacteria in Surface Waters
Rolf A. Deininger JiYoung Lee Arvil Ancheta
School of Public Health The University of Michigan Ann Arbor, Michigan 48109
June 2002
This study was supported in part by the Michigan Great Lakes Protection Fund of the Department of Environmental Quality under grant number GL 00-059. Their support is gratefully acknowledged.
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Table of Contents
Acknowledgements……………………………………………………………………....iii Executive Summary………………………………………………………………………iv List of Tables …………………………………………………………………………….v List of Figures ……………………………………………………………………………vi Statement of the Problem…………………………………………………………………1 Review of the Literature…………………………………………………………………..1 Approach used in this study………………………………………………………………4 Methodology ……………………………………………………………………………..6 Introduction……………………………………………………………………….6 Preparation of the antibody coated magnetic beads……………………………...6 Analysis of Beach Water Samples…………………………………………………8 Concentration of bacteria by serial filtration……………...……………………...8 Selective capture and measurement of E. coli …………………………………..12 Results of the Investigation………………………………………………………………16 Determination of antibodies specificity …………………………………………………19 Results of Pseudomonas testing …..……………………………………………………. 21 Conclusions……………………………………………………………………………....24 Appendix A. Rapid E. coli Test Procedure………………………………………………25 Appendix B. Estimated Cost of the Test………………………………………..……..…27
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Acknowledgements We greatly appreciate the cooperation and support from the health departments of Genesee, Macomb, Monroe and Washtenaw counties. The following persons generously contributed their time. Richard Badics (Washtenaw County Department of Environmental & Infrastructure Services) Bradley J. Bucklin (Washtenaw County Department of Environmental & Infrastructure Services) Elwin Coll (Macomb County Health Department) Richard Fleece (Washtenaw County Department of Environmental & Infrastructure Services) Nickolas C. Hoffman (Genesee County Health Department) Brian McKenzie (Genesee County Health Department) Christopher Westover (Monroe County Health Department) Gary R. White (Macomb County Health Department)

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