中国农业科学 2009,42(8):2633-2642 Scientia Agricultura Sinica
doi: 10.3864/j.issn.0578-1752.2009.08.001
广西普通野生稻的遗传多样性及分布特征
黄 娟
1,2
,杨庆文 ,陈成斌 ,梁世春 ,张万霞 ,乔伟华 ,王家祥
1
2
2
1
1
1
(1 中国农业科学院作物科学研究所,北京 100081;2 广西农业科学院水稻研究所,南宁 530007)
摘要: 【目的】对广西境内普通野生稻居群遗传多样性进行研究,明确广西普通野生稻遗传多样性分布与地 理特征的关系. 【方法】利用 36 对分布于水稻 12 条染色体上的微卫星引物对来自广西 14 个区域(县级行政区域) 的 27 个居群 690 份普通野生稻材料进行遗传多样性分析. 【结果】所有个体平均等位基因数 A=9.86,有效等位基 因数 Ae=5.05,总遗传多样度 Ht=0.74,基因分化系数 Gst=0.49,说明广西普通野生稻遗传多样性丰富,且居群 内与居群间的多样性对总遗传多样性的贡献相当. 通过不同区域遗传多样性检测和聚类分析, 在遗传一致度为 0.5 处可把广西普通野生稻分为 3 个类群. 【结论】结合广西的地形及野生稻居群的分布情况,3 个类群的形成与山脉 的阻隔,水系的分布以及地质结构等影响基因传播途径的特征关系密切. 关键词:普通野生稻;微卫星;遗传多样性;地理特征
Genetic Diversity and the Geographical Characteristics of Wild Rice (Oryza rufipogon Griff) in Guangxi
HUANG Juan1,2, YANG Qing-wen1, CHEN Cheng-bin2, LIANG Shi-chun2 , ZHANG Wan-xia1, QIAO Wei-hua1, WANG Jia-xiang1
(1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 2Institute of Rice Research, Guangxi Academic of Agricultural Sciences, Nanning 530007)
Abstract: 【Objective】 To estimate the genetic diversity of the populations of wild rice (O. rufipogon Griff) in the whole region of Guangxi Zhuang Autonomous Region and reveal their geographically distribution characteristics. 【Method】 Thirty-six SSR loci distributed on the twelve chromosomes of rice were used to study the genetic diversity of 690 wild rice individuals of 27 natural populations from 14 regions in Guangxi. 【Result】 The mean number of allele (A) was 9.86 and the effective number of allele(Ae) was 5.05. The total genetic diversity (Ht) was 0.74 and the coefficient of genetic differentiation (Gst) was 0.49, which means that the populations of O. rufipogon in Guangxi has abundant genetic diversity and the genetic variability among and between the populations has the same contribution to the total diversity. The analysis of clusters showed that all the samples could be clustered into 3 groups at the coefficient of 0.5, and each group has its own geographic characteristics. 【Conclusion】 O. rufipogon populations in the whole region of Guangxi was not only rich in genetic diversity, but also has close relationship with their geographical conditions like mountains, water systems and geology. Key words: Orazy rufipogon; microsatellite; genetic diversity; geographical characters

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