SANTA CRUZ BIOTECHNOLOGY, INC. C15orf58 (S-18): sc-242054 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND C15orf58 is a 385 amino acid protein that is localized to the cytoplasm and belongs to the VTC2 family. C15orf58 functions as a highly efficient GDP-D- glucose phosphorylase and regulates the amount of GDP-D-glucose in cells. The gene encoding C15orf58 maps to human chromosome 15, which encodes more than 700 genes and is about 3% of the human genome. Angelman and Prader-Willi syndromes are associated with loss of function or deletion of genes in the 15q11-q13 region. In the case of Angelman syndrome, this loss is due to inactivity of the maternal 15q11-q13 encoded UBE3A gene in the brain by either chromosomal deletion or mutation. In cases of Prader-Willi syndrome, there is a partial or complete deletion of this region from the paternal copy of chromosome 15. Tay-Sachs disease is a lethal disorder associated with mutations of the HEXA gene, which is encoded by chromo- some 15. Marfan syndrome is associated with chromosome 15 through the FBN1 gene. REFERENCES 1. Hurowitz, G.I., et al. 1993. Neuropsychiatric aspects of adult-onset Tay-Sachs disease: two case reports with several new findings. J. Neuropsychiatry Clin. Neurosci. 5: 30-36. 2. Midla, G.S. 2008. Diagnosis and management of patients with Marfan syndrome. JAAPA 21: 21-25. 3. Dan, B. 2009. Angelman syndrome: current understanding and research prospects. Epilepsia 50: 2331-2339. 4. Ferrer-Bolufer, I., et al. 2009. Tyrosinemia type 1 and Angelman syndrome due to paternal uniparental isodisomy 15. J. Inherit. Metab. Dis. 32: S349-S353. 5. Wawrzik, M., et al. 2010. The C15orf2 gene in the Prader-Willi syndrome region is subject to genomic imprinting and positive selection. Neurogenetics 11: 153-161. CHROMOSOMAL LOCATION Genetic locus: GDPGP1 (human) mapping to 15q26.1; D330012F22Rik (mouse) mapping to 7 D3. SOURCE C15orf58 (S-18) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of C15orf58 of human origin. PRODUCT Each vial contains 200 ?g IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-242054 P, (100 ?g peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. APPLICATIONS C15orf58 (S-18) is recommended for detection of C15orf58 of human origin, D330012F22Rik of mouse origin and the corresponding rat homolog by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 ?g per 100-500 ?g of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- 1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30- 1:3000). C15orf58 (S-18) is also recommended for detection of C15orf58 in additional species, including equine, bovine and porcine. Suitable for use as control antibody for C15orf58 siRNA (h): sc-90100, D330012F22Rik siRNA (m): sc-142810, C15orf58 shRNA Plasmid (h): sc-90100-SH, D330012F22Rik shRNA Plasmid (m): sc-142810-SH, C15orf58 shRNA (h) Lentiviral Particles: sc-90100-V and D330012F22Rik shRNA (m) Lentiviral Particles: sc-142810-V. Molecular Weight of C15orf58: 42 kDa. Positive Controls: Jurkat whole cell lysate: sc-2204. RECOMMENDED SECONDARY REAGENTS To ensure optimal results, the following support (secondary) reagents are recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 (dilution range: 1:2000-1:100,000) or Cruz Marker? compatible donkey anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker? Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) with UltraCruz? Mounting Medium: sc-24941. DATA RESEARCH USE For research use only, not for use in diagnostic procedures. PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and support products. C15orf58 (S-18): sc-242054. Western blot analysis of C15orf58 expression in Jurkat whole cell lysate. 90 K – 55 K – 43 K – 34 K – < C15orf58
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SANTA CRUZ BIOTECHNOLOGY, INC. C15orf58 (S-18): sc-242054 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND C15orf58 is a 385 amino acid protein that is localized to the cytoplasm and belongs to the VTC2 family. C15orf58 functions as a highly efficient GDP-D- glucose phosphorylase and regulates the amount of GDP-D-glucose in cells. The gene encoding C15orf58 maps to human chromosome 15, which encodes more than 700 genes and is about 3% of the human genome. Angelman and Prader-Willi syndromes are associated with loss of function or deletion of genes in the 15q11-q13 region. In the case of Angelman syndrome, this loss is due to inactivity of the maternal 15q11-q13 encoded UBE3A gene in the brain by either chromosomal deletion or mutation. In cases of Prader-Willi syndrome, there is a partial or complete deletion of this region from the paternal copy of chromosome 15. Tay-Sachs disease is a lethal disorder associated with mutations of the HEXA gene, which is encoded by chromo- some 15. Marfan syndrome is associated with chromosome 15 through the FBN1 gene. REFERENCES 1. Hurowitz, G.I., et al. 1993. Neuropsychiatric aspects of adult-onset Tay-Sachs disease: two case reports with several new findings. J. Neuropsychiatry Clin. Neurosci. 5: 30-36. 2. Midla, G.S. 2008. Diagnosis and management of patients with Marfan syndrome. JAAPA 21: 21-25. 3. Dan, B. 2009. Angelman syndrome: current understanding and research prospects. Epilepsia 50: 2331-2339. 4. Ferrer-Bolufer, I., et al. 2009. Tyrosinemia type 1 and Angelman syndrome due to paternal uniparental isodisomy 15. J. Inherit. Metab. Dis. 32: S349-S353. 5. Wawrzik, M., et al. 2010. The C15orf2 gene in the Prader-Willi syndrome region is subject to genomic imprinting and positive selection. Neurogenetics 11: 153-161. CHROMOSOMAL LOCATION Genetic locus: GDPGP1 (human) mapping to 15q26.1; D330012F22Rik (mouse) mapping to 7 D3. SOURCE C15orf58 (S-18) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of C15orf58 of human origin. PRODUCT Each vial contains 200 ?g IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-242054 P, (100 ?g peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. APPLICATIONS C15orf58 (S-18) is recommended for detection of C15orf58 of human origin, D330012F22Rik of mouse origin and the corresponding rat homolog by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 ?g per 100-500 ?g of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- 1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30- 1:3000). C15orf58 (S-18) is also recommended for detection of C15orf58 in additional species, including equine, bovine and porcine. Suitable for use as control antibody for C15orf58 siRNA (h): sc-90100, D330012F22Rik siRNA (m): sc-142810, C15orf58 shRNA Plasmid (h): sc-90100-SH, D330012F22Rik shRNA Plasmid (m): sc-142810-SH, C15orf58 shRNA (h) Lentiviral Particles: sc-90100-V and D330012F22Rik shRNA (m) Lentiviral Particles: sc-142810-V. Molecular Weight of C15orf58: 42 kDa. Positive Controls: Jurkat whole cell lysate: sc-2204. RECOMMENDED SECONDARY REAGENTS To ensure optimal results, the following support (secondary) reagents are recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 (dilution range: 1:2000-1:100,000) or Cruz Marker? compatible donkey anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker? Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) with UltraCruz? Mounting Medium: sc-24941. DATA RESEARCH USE For research use only, not for use in diagnostic procedures. PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and support products. C15orf58 (S-18): sc-242054. Western blot analysis of C15orf58 expression in Jurkat whole cell lysate. 90 K – 55 K – 43 K – 34 K – < C15orf58