EraofmodernBiophysics
TimeScales: fsxps— sxmss
etransfer,Hbonding,waterDNA,RNA,proteinrearrangement,proteinfoldingDNAtranscription
( hrdayyearxByr
celldivisionEarthorganisms,animalmigrationevolution
Knowledge:Interdisciplinary 跨越各学科领域 MathematicsPhysicsChemistryBiologyMedical BioPhysics Biology+Physics Biophysicist is a TRUE Scientist ! Explore to the maximum freedom for doing science!
需要物理与非物理背景人材加入 !
ElementaryparticlesofLife
Universalmolecules:DNA,RNA,protein,ATP Interactionsgivingrisetobioprocess:Central Dogma:DNA RNA protein Biologicalfunctions… Nanomachines:molecularmotors,FoF1ATPase.. Howphysicalandchemicalinteractionsleadto complexfunctionsincells Genenetworks,proteinnetworks…….
Cell Nucleus Chromosome Chromatin
Doublestrandedbiopolymer,2sugar phosphatechains(backbones)twisted aroundeachotherformingaRH(Bform) doublehelix.
basepairs:AT&CG
Play(Torture)withDNA
DNAstretching,elasticity DNAdragreduction DNAthermophoresis DNAcondensation DNAunderexternalfields DNAphotolysis DNAratchetmotion ……..
 Toinvestigatetheconformationalchanges insinglebiomolecules,mayprovide significantinsightintohowthemolecule functions. Howforcesatthemolecularlevelofthe orderofpNunderliethevariedchemistries andmolecularbiologyofgenetic materials
Mechanics/ElasticityofSingleBio molecules
DNAtranscriptionbyRNApolymerase
Bustemanteetal,Nature404,103(2000)
T7DNApolymerase
effectoftemplatetensionpolymerase activity Pausing&arrestduringpolymerase Mechanismofpolymerizationkinetics TuningrateofDNAreplicationwith externalstresses
Physicist'sviewoftheDNAchain
DoublehelixstabilizedbyHbonds(bpinteractions) Polymerofpersistencelength~50nmunderlowforce( 5 pN nm from hydrodynamic drag estimate
DNAcondensation&packing
ComplexcompetitionofDNAelasticity, chargeinteractions,volumeinteractions, solventeffects…..
DNAcondensedbyspermidine
+ H 3N

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